A method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles / 法医学杂志
Journal of Forensic Medicine
;
(6): 435-436, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-983424
ABSTRACT
OBJECTIVE@#To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.@*METHODS@#Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis.@*RESULTS@#There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05).@*CONCLUSION@#It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cepillado Dental
/
ADN
/
Reacción en Cadena de la Polimerasa
/
Repeticiones de Microsatélite
/
Células Epiteliales
/
Genética Forense
/
Mucosa Bucal
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2008
Tipo del documento:
Artículo
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