Expression of FAP and alpha-SMA during the incised wound healing in mice skin / 法医学杂志
Journal of Forensic Medicine
; (6): 405-408, 2009.
Article
en Zh
| WPRIM
| ID: wpr-983512
Biblioteca responsable:
WPRO
ABSTRACT
UNLABELLED@#OBJECTIVE To investigate the time-dependent expression of fibroblast activation protein (FAP) and alpha-smooth muscle actin(alpha-SMA) during the incised wound healing of the skin in mice.@*METHODS@#The expression of FAP and alpha-SMA in incised wound of mice skin was detected by immunohistochemistry and Western blot.@*RESULTS@#By immunohistochemistry, the expression of FAP and alpha-SMA in the normal skin and the skin 1 h after injury maintained at a very low level, but the positive cells expressing FAP and alpha-SMA started to elevate 6 h after injury and reached its peak on 5 d for FAP and on 3 d for alpha-SMA, then gradually decreased to the normal level on 14 d. The expression of FAP and alpha-SMA was observed throughout the wound healing stages 1 d after injuries by Western blot as well with a peak expression occurring on 5 d for FAP and on 3 d for alpha-SMA after injury.@*CONCLUSION@#FAP may be a potentially useful marker for wound age determination and alpha-SMA may be used as an effective indicator for the mid- and late stage incised wound of mice skin. The combination use of FAP and alpha-SMA may be potentially effective indicators for wound age determination.
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Índice:
WPRIM
Asunto principal:
Endopeptidasas
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Piel
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Factores de Tiempo
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Cicatrización de Heridas
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Heridas y Lesiones
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Inmunohistoquímica
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Serina Endopeptidasas
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Distribución Aleatoria
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Western Blotting
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Actinas
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2009
Tipo del documento:
Article