DNA degradation of porcine retinal cells for estimation of postmortem interval / 法医学杂志
Journal of Forensic Medicine
;
(6): 250-252, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-983572
ABSTRACT
OBJECTIVE@#To investigate DNA degradation of porcine retinal cells by single cell gel electrophoresis (SCGE) for estimation of postmortem interval (PMI).@*METHODS@#Degradation of retinal cells was observed by SCGE at 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24h after death respectively, under the environmental conditions of being kept in dark place as well as a controlled temperature of (15 +/- 2) degrees C and humidity of (50 +/- 5)%. The comet pictures were captured by fluorescence microscope and analyzed by single cell gel electrophoresis (IMI 1.0).@*RESULTS@#From 2h to 24h postmortem, the degree of degradation of retinal DNA increased with the prolongation of PMI. The postmortem regression functions of head DNA%, L(T)/L(H), I(T)/I(H) were y = 92.227-5.188 x + 0.019 x2 + 0.001 x3 (R2 = 0.971), y = 0.035e(0.191x) (R2 = 0.947), y = 0.099e(0.264x) (R2 = 0.955), respectively.@*CONCLUSION@#The examination of retinal cell DNA degradation by SCGE is useful for estimating PMI.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cambios Post Mortem
/
Retina
/
Porcinos
/
Factores de Tiempo
/
Procesamiento de Imagen Asistido por Computador
/
ADN
/
Núcleo Celular
/
Ensayo Cometa
/
Patologia Forense
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2010
Tipo del documento:
Artículo
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