Determination of ethyl glucuronide in blood and urine by LC-MS/MS / 法医学杂志
Journal of Forensic Medicine
;
(6): 269-272, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-983577
ABSTRACT
OBJECTIVE@#To develop a method for determining ethyl glucuronide (EtG) in blood and urine by liquid chromatograph coupled with tandem mass spectrometer (LC-MS/MS).@*METHODS@#After blood and urine de-proteined by acetonitrile, the supernate obtained from a centrifuge was analyzed by LC-MS/MS.@*RESULTS@#Determination limit of EtG in both blood and urine was 0.05 pg/mL, with a linear range of 0.10-5.00 microg/mL (r > 0.999). Accuracy in both matrixes was 95%-109%. Inter- and intra-day RSD were less than 12%. The method showed an excellent performance when it was used to analyze authentic blood and urine samples for EtG.@*CONCLUSION@#The method is capable for blood and urine EtG analysis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Biomarcadores
/
Detección de Abuso de Sustancias
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Sensibilidad y Especificidad
/
Etanol
/
Alcoholismo
/
Espectrometría de Masas en Tándem
/
Toxicología Forense
/
Glucuronatos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2010
Tipo del documento:
Artículo
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