Application of multiple polymorphism genetic markers in determination of half sibling sharing a same mother / 法医学杂志
Journal of Forensic Medicine
; (6): 279-300, 2010.
Article
en Zh
| WPRIM
| ID: wpr-983580
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#Determination strategies for half sibling sharing a same mother were investigated through the detection of autosomal and X-chromosomal STR (X-STR) loci and polymorphisms on hypervariable (HV) region of mitochondrial DNA (mtDNA).@*METHODS@#Genomic DNA were extracted from blood stain samples of the 3 full siblings and one dubious half sibling sharing the same mother with them. Fifteen autosomal STR loci were genotyped by Sinofiler kit, and 19 X-STR loci were genotyped by Mentype Argus X-8 kit and 16 plex in-house system. Polymorphisms of mtDNA HV-I and HV-II were also detected with sequencing technology.@*RESULTS@#Full sibling relationship between the dubious half sibling and each of the 3 full siblings were excluded based on the results of autosomal STR genotyping and calculation of full sibling index (FSI) and half sibling index (HIS). Results of sequencing for mtDNA HV-I and HV-II showed that all of the 4 samples came from a same maternal line. X-STR genotyping results determined that the dubious half sibling shared a same mother with the 3 full siblings.@*CONCLUSION@#It is reliable to combine three different genotyping technologies including autosomal STR, X-STR and sequencing of mtDNA HV-I and HV-II for determination of half sibling sharing a same mother.
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Índice:
WPRIM
Asunto principal:
Polimorfismo Genético
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ADN Mitocondrial
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Marcadores Genéticos
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Análisis de Secuencia de ADN
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Secuencias Repetidas en Tándem
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Cromosomas Humanos X
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Hermanos
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Genética Forense
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Genotipo
Límite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2010
Tipo del documento:
Article