Construction and application of fluorescence labeled multiplex typing system for 3 new miniSTR loci / 法医学杂志
Journal of Forensic Medicine
; (6): 282-284, 2010.
Article
en Zh
| WPRIM
| ID: wpr-983581
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish a miniSTR multiplex set including three STR loci unlinked from the CODIS loci: D1S1676, D6S1274 and D17S1299, to generate amplified fragment less than 115 bp in size and to study the genotype of degraded DNA samples.@*METHODS@#After amplification with different fluorescence labeled primers, the amplified products from 100 unrelated individual and 2 highly degraded specimens were analyzed by 310 Genetic Analyzer.@*RESULTS@#Three miniSTR loci were determined by fluorescence-labeled multiplex-PCR technique. Each locus was successfully genotyped in all 100 samples. In D1S1676, D6S1274 and D17S1299 loci, 9, 9, 7 alleles and 27, 23, 18 genotypes were observed respectively. The distribution of genotype for three miniSTR loci in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium. The combined exclusion probability and the combined discrimination power of the three STR loci in Chengdu Han population were 0.9991 and 0.9160 respectively.@*CONCLUSION@#This miniSTR multiplex set could be used in individual identification and paternity test. It also provides a new method in the analysis of degraded DNA sample.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Polimorfismo Genético
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ADN
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China
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Dermatoglifia del ADN
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Cartilla de ADN
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Secuencias Repetidas en Tándem
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Técnicas de Amplificación de Ácido Nucleico
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Alelos
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Genética Forense
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Frecuencia de los Genes
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2010
Tipo del documento:
Article