Comparison of mtDNA extraction from different parts of sarcosaphagous insects / 法医学杂志
Journal of Forensic Medicine
;
(6): 336-339, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-983590
ABSTRACT
OBJECTIVE@#To explore mitochondrial DNA (mtDNA) extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide (CTAB) method.@*METHODS@#Thirteen Lucilia sericata (Meigen) and 13 Nicrophorus fossor (Erichson) were collected from the corpses of rabbits placed on the outdoor lawn in Huhehot district. Four parts (head, chest muscle, legs and wings) of insect were collected, and the mtDNA of all samples were extracted using CTAB method. The purity and concentration were tested using protein and nucleic acid spectrophotometry. The integrity of the extracted mtDNA and PCR products were checked by agarose gel electrophoresis. The PCR products were sequenced and the obtained sequences were imputed into GenBank for comparison.@*RESULTS@#mtDNA were successfully extracted from 10 head samples, 6 legs samples, 4 wing samples and 13 chest muscle samples of the Lucilia sericata (Meigen). Also, mtDNA were successfully extracted from 5 head samples, 8 legs samples, 3 wing samples and 13 chest muscle samples of the Nicrophorus fossor (Erichson).@*CONCLUSION@#mtDNA can be obtained from chest muscle and other parts of sarcosaphagous insects using the improved CTAB method.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Especificidad de la Especie
/
Escarabajos
/
ADN Mitocondrial
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Complejo IV de Transporte de Electrones
/
Dípteros
/
Electroforesis en Gel de Agar
/
Entomología
/
Medicina Legal
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2010
Tipo del documento:
Artículo
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