Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis / 法医学杂志
Journal of Forensic Medicine
; (6): 41-43, 2012.
Article
en Zh
| WPRIM
| ID: wpr-983711
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.@*METHODS@#DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.@*RESULTS@#DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.@*CONCLUSION@#The system established in this study is reliable and more testing sensitive for forensic trace evidence.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Oligonucleótidos
/
ADN
/
Amplificación de Genes
/
Reacción en Cadena de la Polimerasa
/
Reproducibilidad de los Resultados
/
Dermatoglifia del ADN
/
Sensibilidad y Especificidad
/
Cartilla de ADN
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Electroforesis Capilar
/
Secuencias Repetidas en Tándem
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2012
Tipo del documento:
Article