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Changes of CK-MB and HSP 60 in electrical-injuried rats / 法医学杂志
Journal of Forensic Medicine ; (6): 333-336, 2012.
Artículo en Chino | WPRIM | ID: wpr-983756
ABSTRACT
OBJECTIVE@#To investigate the changes of creatine kinase-MB (CK-MB) and heat shock protein 60 (HSP 60) in rats without electric marks after electric injury, to identify the relationship of the CK-MB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury.@*METHODS@#The animal model of electric injury without electric marks was established by alternating current (voltage 110 V). Automatic biochemistry analyzer was used to detect the serum CK-MB and immunohistochemical staining technology was used to analyze the tissues of myocardium and left lobe of liver.@*RESULTS@#The amount of serum CK-MB was increased when the rats were injuried, and reached the peak at 30min. Then the amount of CK-MB began to decrease and showed a slight downward trend in 3-5 h after electric injury, and leveled off at 6 h. Immunohistochemistry staining also showed the changes of HSP 60 of rats' myocardial cells and hepatic cells regularly after electric injury.@*CONCLUSION@#The regular changes of serum CK-MB and tissular HSP 60 in rats can be used to diagnosis electric injury and assess the injury of internal organs after the electric injury without electric marks.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Inmunohistoquímica / Chaperonina 60 / Traumatismos por Electricidad / Forma MB de la Creatina-Quinasa / Hígado / Miocardio Límite: Animales Idioma: Chino Revista: Journal of Forensic Medicine Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Inmunohistoquímica / Chaperonina 60 / Traumatismos por Electricidad / Forma MB de la Creatina-Quinasa / Hígado / Miocardio Límite: Animales Idioma: Chino Revista: Journal of Forensic Medicine Año: 2012 Tipo del documento: Artículo