Establishment of the multiplex genotyping system for 16 SNP loci on mtDNA / 法医学杂志
Journal of Forensic Medicine
;
(6): 47-49, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-983880
ABSTRACT
OBJECTIVE@#To establish a multiplex genotyping system of mtDNA SNP.@*METHODS@#A multiplex analysis system of 16-plex mtDNA SNP loci was established with allele specific PCR and capillary electrophoresis genotyping technology. Fifty samples from unrelated Chinese Han individuals were typed with the multiplex system. The multiplex assay was validated by comparing with the direct sequencing method.@*RESULTS@#The genotypes of all 50 samples were correctly determined by the multiplex system. The optimal genotypic graphs were obtained with an input DNA of 0.5-10 pg, and the typing results were completely consistent with those by direct sequencing method.@*CONCLUSION@#The established multiplex system by allele specific PCR has high sensitivity, operational simplicity and high accuracy. It provides an effective and high output method for mtDNA SNP typing.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
ADN Mitocondrial
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Polimorfismo de Nucleótido Simple
/
Alelos
/
Técnicas de Genotipaje
/
Genotipo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2014
Tipo del documento:
Artículo
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