Correlation between five RNA markers of rat's skin and PMI at different temperatures / 法医学杂志
Journal of Forensic Medicine
;
(6): 245-249, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-983910
ABSTRACT
OBJECTIVE@#To explore the correlation between postmortem interval (PMI) and five RNA markers of rat's skin--β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA(18S rRNA), 5S ribosomal RNA (5S rRNA), and microRNA-203 (miR-203), at different temperatures.@*METHODS@#Eighteen SD rats were randomly divided into three environmental temperature groups 4 °C, 15 °C and 35 °C, respectively. Skin samples were taken at 11 time points from 0 h to 120 h post-mortem. The total RNA was extracted from the skin samples and the five RNA levels were detected by real-time fluorescent quantitative PCR. Proper internal reference was selected by geNorm software. Regression analysis of the RNA markers was conducted by GraphPad software.@*RESULTS@#5S rRNA and miR-203 were most suitable internal references. A good linear relationship between PMI and RNA levels (β-actin and GAPDH) was observed in two groups (4 °C and 15 °C), whereas the S type curve relationship between the expression levels of the two markers (β-actin and GAPDH) and PMI was observed in the 35 °C group. The partial linear relationship between 18S rRNA and PMI was observed in the groups (15 °C and 35 °C).@*CONCLUSION@#Skin could be a suitable material for extracting RNA. The RNA expression levels of β-actin and GAPDH correlate well with PMI, and these RNA markers of skin tissue could be additional indice for the estimation of PMI.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cambios Post Mortem
/
Piel
/
Autopsia
/
Temperatura
/
ARN
/
ARN Ribosómico 18S
/
Análisis de Regresión
/
Actinas
/
Estabilidad del ARN
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2014
Tipo del documento:
Artículo
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