Expression of EIIIA+ fibronectin in incised wound of rat's skin / 法医学杂志
Journal of Forensic Medicine
;
(6): 409-412, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-983937
ABSTRACT
OBJECTIVE@#To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat's skin and injury time.@*METHODS@#The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin samples were taken at the margin of wound. The expression of the EIIIA? fibronectin was detected by immunohistochemistry and Western blotting and the relationship be- tween its expression and injury time was observed. Results The expression of EIIIA+ fibronectin was not observed immediately. The basal cell of skin began to show positive expression 0.5 h after injury. With the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the Western blotting analysis.@*CONCLUSION@#The expression of EIIIA+ fibronectin could be used for estimation of injury time in the early stage of skin injury.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Piel
/
Coloración y Etiquetado
/
Factores de Tiempo
/
Inmunohistoquímica
/
Proteínas
/
Fibronectinas
/
Ratas Sprague-Dawley
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2014
Tipo del documento:
Artículo
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