Analysis of GHB and Its Precursors in Urine and Their Forensic Application / 法医学杂志
Journal of Forensic Medicine
;
(6): 200-203, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-983988
ABSTRACT
OBJECTIVE@#To establish the method to analyze γ-hydroxybutyric acid (GHB) and its precursors 1,4-butanediol (1,4-BD) and gamma-butyrolactone (GBL) in urine through LC-MS/MS and provide evidence for related cases.@*METHODS@#GHB-d6 and MOR-d3 were used as the internal standard. The urine sample was separated by LC after protein precipitation with methanol. The electrospray ion source was for ionization. Each compound was detected through multiple-reaction monitoring (MRM) mode.@*RESULTS@#The limits of detection of GHB and its precursors 1,4-BD and GBL were 0.1, 0.1 and 2 μg/mL. The accuracy was 87.6%-98.1%. The intra-day and inter-day precisions were less than 15% and matrix effects were higher than 80%.@*CONCLUSION@#The method is high sensitive, simple, rapid, specific and with high reliability. This study has provided technical support and basic data for forensic cases involving GHB.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espectrometría de Masas
/
Butileno Glicoles
/
4-Butirolactona
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Ciencias Forenses
/
Espectrometría de Masas en Tándem
/
Hidroxibutiratos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2015
Tipo del documento:
Artículo
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