Establishing a 29 Y-STR Loci Multiplex PCR System / 法医学杂志
Journal of Forensic Medicine
;
(6): 456-461, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-984029
ABSTRACT
OBJECTIVE@#To establish a 29 Y-STR loci multiplex PCR system for investigating the genetic polymorphisms and to assess its application value in forensic science.@*METHODS@#A multiplex PCR system was established using a five color fluorescence labeling 29 Y-STR loci (DYS456, DYS389 I , DYS437, DYS447, DYS389 11, DYS438, DYS522, DYS460, DYS458, DYS622, DYS390, DYS392, DYS448, DYS449, DYS391, Y-GA TA-H4, DYS388, DYS19, DYS385a/b, DYS527a/b, DYS393, DYS459a/b, DYS635, DYS439, DYS570 and DYS627) for multiple amplification and capillary electrophoresis. And its applicability was validated with genetic polymorphism data of 29 Y-STR of unrelated 2,000 male samples in Shandong Han population.@*RESULTS@#A total of 1,981 different haplotypes of 2,000 individuals showed genotype diver- sity between 0.370 0 and 0.965 4. The system provided stable and accurate typing with high sensitivity of 0.05 ng. It satisfied the needs of variety of routine biological samples.@*CONCLUSION@#The 29 Y-STR loci multiplex PCR system could be applied for actual cases and establishment of Y-STR database. In addition, it has great significance in forensic science practices and related research.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polimorfismo Genético
/
Haplotipos
/
ADN
/
Etnicidad
/
China
/
Reproducibilidad de los Resultados
/
Repeticiones de Microsatélite
/
Cromosomas Humanos Y
/
Ciencias Forenses
/
Pueblo Asiatico
Límite:
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2015
Tipo del documento:
Artículo
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