A study on pathological changes of closed diffuse brain injury in rats with H.E., esterification-silver stain and albumin immunohistochemical stain / 法医学杂志

ABSTRACT

OBJECTIVE@#To observed the pathological changes of closed diffuse brain injury in the rats died immediately and 15 min to 5 days after the injury.@*METHODS@#H.E. staind and esterification-silver stain were applied to investigate the closed diffuse brain injury.@*RESULTS@#In rats died immediately after the concussive injury, a number of shrunken neurons(type I change), distended neurons(type II change) and wave-like nerve fibers were identified in the brain tissue, especially in brain-stem. At 2 h and 8 h after injury, brain edema and axonal swelling appeared clearly in the cortex and white matter, especially in brain-stem. At post-traumatic 8 h and 24 h, the axonal retraction balls began to appear. The amount of neurons undergoing type I and II changes and constraction balls increased along with the survivor time. After 4 days and 5 days, brain edema alleviated, but the retraction balls and axonal swelling still existed. With Esterifica-tion silver stain, the above changes of neurons and nerve fibers were more obvious. With ABC stain, the distribution of albumin(Al) was extended from the perio-vascular area to diffuse distribution. Al positive staining were more obvious in injuried neurons and nerve fibers.@*CONCLUSION@#The distribution of the concussive damage in the brain are coup, contra-coup and centripental.