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Correlation between RNA Expression Level and Early PMI in Human Brain Tissue / 法医学杂志
Journal of Forensic Medicine ; (6): 245-249, 2016.
Artículo en Chino | WPRIM | ID: wpr-984839
ABSTRACT
OBJECTIVES@#To explore the correlation between the expression levels of several RNA markers in human brain tissue and early postmortem interval (PMI).@*METHODS@#Twelve individuals with known PMI (range from 4.3 to 22.5 h) were selected and total RNA was extracted from brain tissue. Eight commonly used RNA markers were chosen including β-actin, GAPDH, RPS29, 18S rRNA, 5S rRNA, U6 snRNA, miRNA-9 and miRNA-125b, and the expression levels were detected in brain tissue by real-time fluorescent quantitative PCR. The internal reference markers with stable expression in early PMI were screened using geNorm software and the relationship between its expression level and some relevant factors such as age, gender and cause of death were analyzed. RNA markers normalized by internal reference were inserted into the mathematic model established by previous research for PMI estimation using R software. Model quality was judged by the error rate calculated with estimated PMI.@*RESULTS@#5S rRNA, miRNA-9 and miRNA-125b showed quite stable expression and their expression levels had no relation with age, gender and cause of death. The error rate of estimated PMI using β-actin was 24.6%, while GAPDH was 41.0%.@*CONCLUSIONS@#5S rRNA, miRNA-9 and miRNA-125b are suitable as internal reference markers of human brain tissue owing to their stable expression in early PMI. The expression level of β-actin correlates well with PMI, which can be used as an additional index for early PMI estimation.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cambios Post Mortem / Autopsia / Programas Informáticos / Encéfalo / ARN Ribosómico 5S / ARN Ribosómico 18S / ARN Nuclear Pequeño / Actinas / Estabilidad del ARN / MicroARNs Límite: Humanos Idioma: Chino Revista: Journal of Forensic Medicine Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cambios Post Mortem / Autopsia / Programas Informáticos / Encéfalo / ARN Ribosómico 5S / ARN Ribosómico 18S / ARN Nuclear Pequeño / Actinas / Estabilidad del ARN / MicroARNs Límite: Humanos Idioma: Chino Revista: Journal of Forensic Medicine Año: 2016 Tipo del documento: Artículo