Review of Second Generation Sequencing and Its Application in Forensic Genetics / 法医学杂志
Journal of Forensic Medicine
;
(6): 282-289, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-984849
ABSTRACT
The rapid development of second generation sequencing (SGS) within the past few years has led to the increasement of data throughput and read length while at the same time brought down substantially the sequencing cost. This made new breakthrough in the area of biology and ushered the forensic genetics into a new era. Based on the history of sequencing application in forensic genetics, this paper reviews the importance of sequencing technologies for genetic marker detection. The application status and potential of SGS in forensic genetics are discussed based on the already explored SGS platforms of Roche, Illumina and Life Technologies. With these platforms, DNA markers (SNP, STR), RNA markers (mRNA, microRNA) and whole mtDNA can be sequenced. However, development and validation of application kits, maturation of analysis software, connection to the existing databases and the possible ethical issues occurred with big data will be the key factors that determine whether this technology can substitute or supplement PCR-CE, the mature technology, and be widely used for cases detection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN Mitocondrial
/
ARN Mensajero
/
Marcadores Genéticos
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Polimorfismo de Nucleótido Simple
/
MicroARNs
/
Genética Forense
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2016
Tipo del documento:
Artículo
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