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Application of Multiple Displacement Amplification in Samples with Inhibitors / 法医学杂志
Journal of Forensic Medicine ; (6): 342-345, 2016.
Artículo en Chino | WPRIM | ID: wpr-984857
ABSTRACT
OBJECTIVES@#To explore the ability of inhibition resistibility of multiple displacement amplification (MDA) in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods (MBM) to purify sample.@*METHODS@#Different concentrations of hemoglobin and humid acid (HA) mixed with DNA samples and then divided the samples into MDA group, MBM group and control group. D3S1358 locus was amplified and detected by polyacrylamide gel electrophoresis detection system and AmpFℓSTR® Identifiler™ Plus Kit-capillary electrophoresis detection system.@*RESULTS@#When hemoglobin concentrations exceed 1 ng/μL or HA concentrations exceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.@*CONCLUSIONS@#MDA has the capability to remove the inhibition of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ADN / Hemoglobinas / Técnicas de Amplificación de Ácido Nucleico / Electroforesis en Gel de Poliacrilamida / Medicina Legal / Sustancias Húmicas Límite: Humanos Idioma: Chino Revista: Journal of Forensic Medicine Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ADN / Hemoglobinas / Técnicas de Amplificación de Ácido Nucleico / Electroforesis en Gel de Poliacrilamida / Medicina Legal / Sustancias Húmicas Límite: Humanos Idioma: Chino Revista: Journal of Forensic Medicine Año: 2016 Tipo del documento: Artículo