Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS / 法医学杂志
Journal of Forensic Medicine
; (6): 516-523, 2021.
Article
en Zh
| WPRIM
| ID: wpr-985242
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid screening and testing of three types of new psychoactive tryptamines in human blood: 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT. Methods The effects of the type of extractant, the type and dosage of salting-out agent, and the dosage of adsorbent on the test results of the three tryptamines were investigated. Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS. Results The linear relationships of 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100, 0.5-100 and 0.2-100 ng/mL, respectively, with their coefficients higher than 0.99. The limits of detection (LODs) were 0.1-0.2 ng/mg. The recoveries ranged from 84.86% to 94.57%. Intra-day and inter-day precisions were good. Conclusion The method is simple, rapid, easy to operate and has a high recovery. It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.
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Índice:
WPRIM
Asunto principal:
Triptaminas
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Cromatografía Líquida de Alta Presión
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Cromatografía Liquida
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Espectrometría de Masas en Tándem
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Límite de Detección
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2021
Tipo del documento:
Article