Rapid determination of acetaminophen in plasma by LC-MS/MS / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 364-366, 2023.
Artículo
en Chino
| WPRIM
| ID: wpr-986014
ABSTRACT
Objective:
To establish a method for the rapid determination of acetaminophen (APAP) in human plasma by LC-MS/MS.Methods:
The plasma samples were extracted by methanol and acetonitrile (1 1) and purified directly. C(18) column was used for sample separation. The mobile phase were methanol (5 mmol/L ammonium acetate) and water (5 mmol/L ammonium acetate). Samples were analyzed by LC MS/MS with the electrospray ionization multi reaction monitoring (MRM) mode.Results:
The calibration curves of APAP was linear in the concentration range of 0~10 mg/L, the correlation coefficient (r) was greater than 0.999 0. The relative standard deviation within and between batches was less than 10%. The recovery rate were 96.81%~101.7%. The detection limit of the method was 0.1 μg/L and the lower limit of quantification was 0.3 μg/L.Conclusion:
This method has strong specificity, high sensitivity and reliable determination results. It is suitable for the rapid analysis of clinical plasma samples.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Cromatografía Liquida
/
Metanol
/
Espectrometría de Masas en Tándem
/
Acetaminofén
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2023
Tipo del documento:
Artículo
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