Dexamethasone effects on survival, differentiation, and proliferation of peritoneal (or B-1 cells) and splenic B cells / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 73-81, 2008.
Artículo
en Coreano
| WPRIM
| ID: wpr-98959
ABSTRACT
OBJECTIVE:
Although the characteristic feature and function of peritoneal B (B-1) cells are very different from splenic B (B-2) cells, peritoneal B cell study is not known well. The aim of this study is to investigate the effects of dexamethasone on peritoneal (or B-1 cells) and splenic B cells (or B-2 cells).METHODS:
The synthetic corticosteroid, dexamethasome, was injected to BALBc mice intraperitoneally or subcutaneously at 4-5 pm for 7 days. Expression level of B cell surface marker analyzed by flow cytometry. The purified peritoneal B cell and splenic B cells were obtained and cell survival rate was analysed by flow cytometry. Isolated B cells were cultured in medium with different concentration of dexamaethasone. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. Entering of S phase was measured by proliferative assay.RESULTS:
Subcutaneous injection of high dose of Dexamethasone for 7 days did not affect cell surface markers of peritoneal and splenic B cells. However, all cell surface markers of peritoneal B cell after the treatment of dexamethasone are reduced by daily intraperitoneal injection of dexamethasone for 2 days. The survival rate of peritoneal and splenic B cell decrease with increasing concentration of dexamethasone. Proliferation of peritoneal B cells was less affected by dexamethasone than that of splenic B cells (p<0.05).CONCLUSIONS:
Dexamethasone induced apoptosis in both peritoneal and splenic B cells. Proliferation and differentiation of splenic B cells were affected by dexamethasone, but peritoneal B cells are less sensitive to dexamethasone.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunoglobulinas
/
Dexametasona
/
Ensayo de Inmunoadsorción Enzimática
/
Linfocitos B
/
Supervivencia Celular
/
Tasa de Supervivencia
/
Fase S
/
Apoptosis
/
Citometría de Flujo
/
Inyecciones Intraperitoneales
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2008
Tipo del documento:
Artículo
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