Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046
International Journal of Oral Biology
;
: 79-82, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-9938
ABSTRACT
The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Esguinces y Distensiones
/
ADN
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Prevotella intermedia
/
Prevotella
/
Límite de Detección
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
International Journal of Oral Biology
Año:
2011
Tipo del documento:
Artículo
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