The role of ischemic preconditioning in the expression of apoptosis-related genes in a rat model of intestinal ischemia-reperfusion injury
Acta cir. bras
;
34(5): e201900501, 2019. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-1010875
ABSTRACT
Abstract Purpose:
To analyze the effects of ischemic preconditioning (IPC) in the expression of apoptosis-related genes in rat small intestine subjected to ischemia and reperfusion.Methods:
Thirty anesthetized rats underwent laparotomy and were drive into five groups control (CG); ischemia (IG); ischemia and reperfusion (IRG); IPC and ischemia (IG+IPC); IPC and ischemia and reperfusion (I/RG+IPC). Intestinal ischemia was performed by clamping the superior mesenteric artery for 60 minutes, whereas reperfusion lasted for 120 minutes. IPC was carried out by one cycle of 5 minutes of ischemia followed by 10 minutes of reperfusion prior to the prolonged 60-minutes-ischemia and 120-minutes-reperfusion. Thereafter, the rats were euthanized and samples of small intestine were processed for histology and gene expression.Results:
Histology of myenteric plexus showed a higher presence of neurons presenting pyknotic nuclei and condensed chromatin in the IG and IRG. IG+IPC and I/RG+IPC groups exhibited neurons with preserved volume and nuclei, along with significant up-regulation of the anti-apoptotic protein Bcl2l1 and down-regulation of pro-apoptotic genes. Moreover, Bax/Bcl2 ratio was lower in the groups subjected to IPC, indicating a protective effect of IPC against apoptosis.Conclusion:
Ischemic preconditioning protect rat small intestine against ischemia/reperfusion injury, reducing morphologic lesions and apoptosis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Lésion d'ischémie-reperfusion
/
Apoptose
/
Préconditionnement ischémique
/
Protéines régulatrices de l'apoptose
/
Jéjunum
Type d'étude:
Études d'évaluation
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2019
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
UNIFESP/BR
/
Universidade Estadual do Piauí/BR
/
Universidade Federal de São Paulo/BR
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