IRF2-INPP4B-mediated autophagy suppresses apoptosis in acute myeloid leukemia cells
Biol. Res
;
52: 11, 2019. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1011413
ABSTRACT
BACKGROUND:
The present study aimed to investigate the underlying role of interferon-regulatory factor 2 (IRF2)-inositol polyphosphate-4-phosphatase, type-II (INPP4B) axis in the regulation of autophagy in acute myeloid leukemia (AML) cells.METHODS:
Quantitative real time PCR (QRT-PCR) and western blot were performed to determine the expression levels of IRF2, INPP4B and autophagy-related markers in AML cell lines. Autophagy was assessed by elevated Beclin-1 expression, the conversion of light chain 3 (LC3)-I to LC3-II, downregulated p62 expression and green fluorescent protein (GFP)-LC3 puncta formation. The colony formation and apoptosis assays were performed to determine the effects of IRF2 and INPP4B on the growth of AML cells.RESULTS:
IRF2 and INPP4B were highly expressed in AML cell lines, and were positively correlated with autophagy-related proteins. Overexpression of IRF2 or INPP4B stimulated autophagy of AML cells, whereas inhibition of IRF2 or INPP4B resulted in the attenuation of autophagy. More importantly, IRF2 or INPP4B overexpression reversed autophagy inhibitor, 3-methyladenine (3-MA)-induced proliferation-inhibitory and pro-apoptotic effects, while IRF2 or INPP4B silencing overturned the proliferation-promoting and anti-apoptotic effects of autophagy activator rapamycin.CONCLUSION:
IRF2-INPP4B signaling axis attenuated apoptosis through induction of autophagy in AML cells.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Autophagie
/
Leucémie aigüe myéloïde
/
Apoptose
/
Phosphoric monoester hydrolases
/
Facteur-2 de régulation d'interféron
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2019
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
The First Affiliated Hospital of Bengbu Medical College/CN
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