Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
Biol. Res
;
52: 31, 2019. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1019497
ABSTRACT
BACKGROUND:
The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells.METHODS:
The levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues and CNE2 cells were determined by methylation-specific PCR and RT-PCR, respectively. CNE2 cells were treated with 5-aza-2-deoxycytidine, and the variation in the methylation status of the DACT1 gene was detected, as well as the influence of methylation on invasiveness of nasopharyngeal carcinoma cells.RESULTS:
The DACT1 gene was hyper-methylated in 44 of 62 cases of nasopharyngeal carcinoma. The DACT1 gene was hyper-methylated in 32 of 38 cases of nasopharyngeal carcinoma with lymph node metastasis, and the DACT1 gene was hyper-methylated in 7 of 24 cases of nasopharyngeal carcinoma without lymph node metastasis. The DACT1 mRNA level was weakly expressed or not expressed in all nasopharyngeal carcinoma tissues with hyper-methylated DACT1 genes; however, the DACT1 mRNA level was highly expressed in nasopharyngeal carcinoma tissues with low expression of the methylated DACT1 gene. The DACT1 gene was hyper-methylated and not expressed in CNE2 cells that did not have 5-aza-2-deoxycytidine treatment. After 5-aza-2-deoxycytidine treatment, the DACT1 gene was demethylated and the expression of DACT1 was restored. Moreover, the invasion ability was inhibited in CNE2 cells treated with 5-aza-2-deoxycytidine.CONCLUSION:
The expression of DACT1 was related to the methylation status. High expression of DACT1 may inhibit the invasion and metastasis of nasopharyngeal carcinoma cells.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Protéines nucléaires
/
Tumeurs du rhinopharynx
/
Méthylation de l'ADN
/
Protéines adaptatrices de la transduction du signal
/
Cancer du nasopharynx
Limites du sujet:
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2019
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
University of Chinese Academy Sciences-Shenzhen Hospital/CN
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