Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine
J. venom. anim. toxins incl. trop. dis
;
23: 28, 2017. graf
Article
Dans Anglais
| LILACS, VETINDEX
| ID: biblio-1040378
ABSTRACT
Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. Methods In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. Results We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, in contrast to the antivenom raised against crotamine-negative venom. Conclusions These results indicate that venomic databases and antivenomics analysis provide a useful approach for choosing the better venom mixture for antibody production and for the subsequent screening of antivenom cross-reactivity with relevant snake venom components.(AU)
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Morsures et piqûres
/
Sérums antivenimeux
/
Crotalus cascavella
/
Venins de crotalidé
/
Production d'anticorps
Type d'étude:
Guide de pratique
langue:
Anglais
Texte intégral:
J. venom. anim. toxins incl. trop. dis
Année:
2017
Type:
Article
Institution/Pays d'affiliation:
Instituto Vital Brazil/BR
/
Universidade Federal do Rio de Janeiro/BR
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