Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
Mem. Inst. Oswaldo Cruz
;
112(2): 155-159, Feb. 2017. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-1040566
ABSTRACT
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. FINDINGS Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. MAIN CONCLUSION Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Leishmania brasiliensis
/
Amphotéricine B
/
Protéines à fluorescence verte
/
Évaluation préclinique de médicament
/
Antiprotozoaires
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2017
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Viçosa/BR
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