Staphylococcus aureus resistente a meticilina en trabajadores del centro integral de salud de la Universidad Católica Santo Toribio e d Mogrovejo- Chiclayo 2009 / Methicillin resistant Staphylococcus aureus in workers of integral health center of The Catholic University Santo Toribio e Mogrovejo- Chiclayo 2009

RESUMEN

Objetivo: Determinar la frecuencia de S. aureus resistente a Meticilina (SARM) en trabajadores del Centro Integral de Salud de la Universidad Catolica Santo Toribio de Mogrovejo. El estudio Se incluyo en el estudio al total de trabajadores del Centro (n=35). A cada uno se le realizo dos hisopados, uno en las fosas nasales y otro en la faringe. Las muestras se sembraron en placas con agar sangre y manitol salado. Se incubaron en atmosfera de CO2 a 37oC por 24 horas. Se realizo coloracion Gram, prueba de catalasa y coagulasa. Para el antibiograma, se utilizo el metodo de Kirby Bauer (CLSI). Los antibioticos evaluados fueron ciprofloxacina (5ug), clindamicina (2ug), eritromicina (15ug), levofloxacina (5ug), oxacilina (1ug) y vancomicina (30ug). Hallazgos Se aislo 8 cepas de S. aureus sensibles a meticilina y 6 cepas de Streptococcus pyogenes -hemolitico. Conclusiones: No hubo prevalencia de S. aureus resistente a Meticilina en los trabajadores del Centro Integral de Salud de la Universidad Catolica Santo Toribio de Mogrovejo.(AU)

ABSTRACT

To determine the prevalence of methicillinresistant S. aureus in workers of Integral Health Center of the Catholic University Santo Toribio de Mogrovejo. The study All employees of the Center were included (n = 35). Each worker underwent two swabs, one in the nose and another in the pharynx. Samples were inoculated in blood agar and manitol salt plates. The plates were incubated in a CO2 atmosphere at 37°C for 24 hours. Gram staining, catalase and coagulase test were performed. For antibiotic susceptibility testing, we used the method of Kirby Bauer (CLSI). The antibiotics evaluated were ciprofloxacin (5ìg), clindamycin (2ìg), erythromycin (15ìg), levofloxacin (5ìg), oxacilin (1ìg), vancomycin (30ìg). Findings: 8 strains of methicillinsensitive S. aureus and 6 strains of â-hemolytic Streptococcus pyogenes were isolated. Conclusions: There was no prevalence of methicillin-resistant S. aureus in workers Integral Health Center at the Catholic University Santo Toribio de Mogrovejo. Control of risk factors, biosecurity measures implemented and the participation of all workers in a Health Center, contribute to the monitoring of MRSA.(AU)