Productive infection and transduction by bacteriophage P1 in the species Salmonella bongori
Electron. j. biotechnol
;
41: 9-12, sept. 2019. tab, ilus
Article
Dans Anglais
| LILACS
| ID: biblio-1053556
ABSTRACT
Background:
Horizontal gene transfer (HGT) is the most important mechanism in the evolution of new genetic capabilities in bacteria, including specific degradative pathways, virulence factors, and resistance to antibiotics. Among the processes involved in HGT, transduction is noteworthy. This is a mechanism for gene transmission mediated by a bacteriophage that functions both as a reservoir and as a vector of exogenous genes, which remain protected from environmental effects in the bacteriophage's capsid. Within this context, this investigation aimed to evaluate the ability of the generalized transducing bacteriophage P1 to productively infect and transduce in the bacterial species Salmonella bongori.Results:
We could establish that a derivative of bacteriophage P1, P1Cm, infects strains of S. bongori with frequencies of lysogenization in the order of ~10−2 lysogens/UFP. Through thermal induction, infective viral progeny was obtained, and we could show that P1Cm readily formed plaques on S. bongori lawns, a phenomenon thus far not reported for other members of the genus Salmonella. Finally, we showed P1Cm-mediated transduction of the model plasmid RP4 at frequencies of ~10−7 transductants/donor.Conclusion:
Therefore, bacteriophage P1 can be used as a tool for the genetic manipulation in the species S. bongori.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Salmonella
/
Transduction génétique
/
Bactériophage P1
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
Electron. j. biotechnol
Thème du journal:
Biotechnologie
Année:
2019
Type:
Article
Pays d'affiliation:
Canada
/
Chili
Institution/Pays d'affiliation:
Memorial University of Newfoundland/CA
/
Pontificia Universidad Católica de Valparaíso/CL
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