Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells
Braz. j. med. biol. res
;
53(5): e9331, 2020. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1098113
ABSTRACT
The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co‐exposure in human kidney 293 cells using the MTT assay. During a 24-h period for the three concentrations tested (0.5, 1, and 5 mg/mL), neither melamine nor CA alone showed significant toxic effects on 293 cells at 0.5 mg/mL, while higher concentrations led to decreased in cell viability. However, co-exposure to several combinations of melamine and CA [1001, 101, 110, and 1100 (vv), at a final concentration of 0.5 mg/mL] did cause cytotoxicity with higher levels of CA leading to higher cytotoxicity. By contrast, while neither melamine nor CA alone induced phosphorylated-H2AX (γH2AX) foci formation, melamine and CA at a 1001 ratio induced γH2AX foci 24 h post-treatment. The alkaline comet assay also revealed the presence of DNA damage following melamine and CA co-exposure. In vivo assay also revealed the presence of melamine-CA complex in the kidney. These data indicated that the cytotoxic and genotoxic effects of melamine and CA co-exposure differ from those of melamine or CA alone.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Triazines
/
Altération de l'ADN
/
Survie cellulaire
/
Rein
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2020
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Hangzhou Normal University School of Medicine/CN
/
Hospital Infection Control Department, Zhejiang Provincial TCM Hospital/CN
/
Zhejiang University School of Medicine/CN
/
Zhejiang University School of Public Health/CN
/
Zhejiang-California International Nanosystems Institute/CN
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