Mechanical properties of the sciatic nerve following combined transplantation of analytically extracted acellular allogeneic nerve and adipose-derived mesenchymal stem cells
Acta cir. bras
;
35(4): e202000405, 2020. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-1130632
ABSTRACT
Abstract Purpose To investigate the effects of Chemically Extracted Acellular Nerves (CEANs) when combined with Adipose-Derived mesenchymal Stem Cell (ADSC) transplantation on the repair of sciatic nerve defects in rabbits. Methods A total of 71 six-month-old Japanese rabbit were used in this study. Twenty rabbits served as sciatic nerve donors, while the other 51 rabbits were randomly divided into Autologous Nerve Transplantation Group (ANT, n=17), CEAN group (n=17) and CEAN-ADSCs group (n=17). In all these groups, the rabbit's left sciatic nerves were injured before the experiment, and the uninjured sciatic nerves on their right side were used as the control (CON). Electrophysiological tests were carried out and sciatic nerves were prepared for histomorphology and stretch testing at 24 weeks post-transplant. Results There were significant differences between ANT and Con groups in amplitude (AMP) P=0.031; motor nerve conduction velocity (MNCV) P=0.029; Maximum stress P=0.029; and Maximum strain P=0.027. There were also differences between the CEAN and CEAN+ADSCs groups in AMP P=0.026, MNCV P=0.024; Maximum stress P=0.025 and Maximum strain P=0.030. No significant differences in these parameters were observed when comparing the ANT and CEAN+SACN groups (MNCV P=0.071) or the CEAN and ANT groups (Maximum stress P=0.069; Maximum strain P=0.077). Conclusion Addition of ADSCs has a significant impact on the recovery of nerve function, morphology, and tensile mechanical properties following sciatic nerve injury.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Neuropathie du nerf sciatique
/
Transplantation de cellules souches mésenchymateuses
/
Cellules souches mésenchymateuses
/
Tissu nerveux
Type d'étude:
Études d'évaluation
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2020
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Changchun University of Chinese Medicine/CN
/
Jilin University/CN
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