Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury
Acta cir. bras
;
35(9): e202000901, 2020. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1130681
ABSTRACT
Abstract Purpose To evaluate the effects of treatment with Indigo Carmine (IC) on rat livers subjected to ischemia-reperfusion injury. Methods The animals were subdivided into 4 groups 1.SHAM group(SH) - saline; 2.SHAM group with IC-2mg/Kg(SHIC); 3.IR group - rats submitted to ischemia and reperfusion with saline(IR); 4.IR group with IC-2mg/Kg(IRIC). The IR protocol consists of liver exposure and administration of drug or saline intravenously, followed by 60 minutes of ischemia and 15 of reperfusion. Liver samples were collected for biochemical analysis. Results State 3 of mitochondrial respiration showed a significant worsening of the IRIC group in relation to all others. State 4 showed a difference between IRIC and SHIC. The Respiratory Control Ratio showed statistical decrease in IR and IRIC versus Sham. The osmotic swelling showed significant difference between SHxIR; SHICxIRIC and SHxIRIC. There was a significant increase in ALT in the IRIC group in relation to all the others. Concerning the nitrate dosage, there was a decrease in the group treated with IC(IRxIRIC). There was no difference regarding the dosage of Malondialdehyde. Conclusion IC was not able to protect mitochondria from IR injury and proved to be a potentiating agent, acting in synergy with the IR injury promoting damage to the hepatocyte membranes.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Lésion d'ischémie-reperfusion
/
Carmin d'indigo
/
Ischémie
Type d'étude:
Guide de pratique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2020
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Faculdade de Medicina de Marília/BR
/
USP/BR
/
Universidade de São Paulo/BR
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