Risk Factors for Early Childhood Caries Based on Identification of Veillonella spp. Using RT-PCR
Pesqui. bras. odontopediatria clín. integr
;
20: e5701, 2020. tab
Article
Dans Anglais
| LILACS, BBO
| ID: biblio-1135530
ABSTRACT
Abstract Objective:
To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material andMethods:
A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test.Results:
A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83.Conclusion:
The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Veillonella
/
Réaction de polymérisation en chaîne
/
Facteurs de risque
/
Caries dentaires
/
Bactéries à Gram négatif
Type d'étude:
Etude diagnostique
/
Etude d'étiologie
/
Étude observationnelle
/
Étude de prévalence
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Enfant d'âge préscolaire
/
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Pesqui. bras. odontopediatria clín. integr
Thème du journal:
Dentisterie
Année:
2020
Type:
Article
Pays d'affiliation:
Indonésie
Institution/Pays d'affiliation:
University of Indonesia/ID
/
University of Padjadjaran/ID
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