Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells
Pesqui. bras. odontopediatria clín. integr
;
20: e0044, 2020. tab, graf
Article
Dans Anglais
| LILACS, BBO
| ID: biblio-1135549
ABSTRACT
Abstract Objective:
To examine the cytotoxicity of calcium hydroxide on human umbilical cord mesenchymal stem cells (HUCMSC) to understand the characteristics for use in regenerative dentistry procedures especially regenerative endodontics. Material andMethods:
HUCMSC was isolated, cultured, and confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSC was cultured and divided into two groups, the control group (cultured in minimum essential medium (MEM) alpha) and calcium hydroxide group (cultured in MEM alpha and calcium hydroxide). Methyl-thiazole-tetrazolium (MTT) assay was done on different concentrations of calcium hydroxide (0.39 to 25 µg/mL) and the cells were observed and counted. One-way ANOVA test was used with a significance level set at 5%.Results:
Flow cytometric analysis confirmed positive of CD73, CD90, CD105, negative of CD45 and CD34. A significant difference was found between the concentration of 6.25 and 3.125 µg/mL (p=0.004). There was no significant difference among 6.25, 12.5 and 25 µg/mL concentrations. There was also no significant difference among 0.39, 0.78, 1.56, and 3.125 µg/mL concentrations.Conclusion:
Even though calcium hydroxide is a medicament of choice in clinical endodontics, it decreases the viability of HUCMSC. The lower the concentration of calcium hydroxide, the higher the viability of HUCMSC.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Hydroxyde de calcium
/
Survie cellulaire
/
Recherche sur les cellules souches
/
Cellules souches mésenchymateuses
/
Endodontie régénératrice
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Pesqui. bras. odontopediatria clín. integr
Thème du journal:
Dentisterie
Année:
2020
Type:
Article
Pays d'affiliation:
Indonésie
Institution/Pays d'affiliation:
Universitas Airlangga/ID
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