ADAMTS proteoglycanases downregulation with impaired oocyte quality in PCOS
Arch. endocrinol. metab. (Online)
;
65(1): 60-66, Jan.-Feb. 2021. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-1152880
ABSTRACT
ABSTRACT Objective:
A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) and ADAMTS-5 normal expression levels are essential for ovulation and subsequent fertilization. The objective of the present study was to assess expression pattern of these genes in cumulus cells (CCs) taken from patients with polycystic ovary syndrome (PCOS) and to investigate any possible relationship with the oocyte quality. Subjects andmethods:
ADAMTS-4 and -5 expression levels within CCs containing oocytes at the metaphase II (MII) and germinal vesicle (GV) stages, taken from 35 patients with PCOS and 35 women with normal ovarian function, were investigated using RT-qPCR. Moreover, possible correlations between ADAMTS-4, ADAMTS-5, and progesterone receptors (PRs) expression as well as oocyte quality were evaluated.Results:
ADAMTS-4 and -5 expression levels were dramatically diminished in the CCs of the PCOS patients when compared to the controls. ADAMTS-4 and -5 expression levels were correlated with each other and with the oocyte quality. Furthermore, lower expression levels of ADAMTS-4 and -5 in the PCOS patients were strongly correlated with the diminished PRs expression levels.Conclusions:
Downregulation of ADAMTS-4 and -5 in the human CCs of the PCOS patients correlated with the decline in the PRs expression, and impaired oocyte quality may cause lower oocyte recovery, maturation, and fertilization rate.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Ovocytes
/
Syndrome des ovaires polykystiques
/
Protéine ADAMTS4
/
Protéine ADAMTS5
Limites du sujet:
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Arch. endocrinol. metab. (Online)
Thème du journal:
Endocrinologie
/
Métabolisme
Année:
2021
Type:
Article
Pays d'affiliation:
Iran
Institution/Pays d'affiliation:
Hamadan University of Medical Sciences/IR
/
School of Medicine/IR
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