Identificación de especies de Malassezia por PCR-REA / [Identification of Malassezia species by PCR-REA]
Rev. argent. microbiol
;
35(3): 162-6, 2003 Jul-Sep.
Article
Dans Espagnol
|
LILACS-Express
| LILACS, BINACIS
| ID: biblio-1171720
ABSTRACT
The genus Malassezia has acquired relevance in the last years for its pathological associations. Since 1996, the genus comprises M. pachydermatis, M. furfur, M. sympodialis, M. slooffiae, M. obtusa, M. globosa and M. restricta. The identification scheme based on morphological, physiological and biochemical characteristics does not resolve ambiguity between some species. The aim of this study was to find out a fast, specific and efficient methodology to distinguish between Malassezia species. Based on a previous technique for polymerase chain reaction-restriction enzyme analysis (PCR-REA), modifications were applied on DNA extraction and purification and the electrophoresis. These changes produced a cheaper, reliable and rapid method as identification procedure with easily defined results. The proposed modifications would allow a better knowledge on ecological and pathogenic roles of Malassezia, studies that have not yet been established.
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Indice:
LILAS (Amériques)
Type d'étude:
Etude diagnostique
/
Étude pronostique
langue:
Espagnol
Texte intégral:
Rev. argent. microbiol
Thème du journal:
Microbiologie
Année:
2003
Type:
Article
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