Oxidized low-density lipoprotein stimulates dendritic cells maturation via LOX-1-mediated MAPK/NF-κB pathway
Braz. j. med. biol. res
;
54(9): e11062, 2021. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-1249335
ABSTRACT
Dendritic cells (DCs) play a crucial role as central orchestrators of immune system response in atherosclerosis initiation and progression. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is involved in the immune maturation of DCs, but the underlying mechanisms remain unclear. We isolated mouse bone marrow progenitors and stimulated them with granulocyte-macrophage colony-stimulating factor and interleukin (IL)-4 to induce immature DCs. We then treated DCs with oxidized low-density lipoprotein (oxLDL) to induce maturation. LOX-1 siRNA was used to investigate the modulation of LOX-1 on the development of DCs and the underlying signal pathways. CD11c-positive DCs were successfully derived from mouse bone marrow progenitors. OxLDL promoted the expressions of DCs maturation markers and pro-inflammatory cytokines. OxLDL also upregulated LOX-1 expression and activated MAPK/NF-κB pathways. LOX-1 siRNA could attenuate the expression of MAPK/NF-κB pathways and inflammatory cytokines. In conclusion, oxLDL induced the maturation of DCs via LOX-1-mediated MAPK/NF-κB pathway, which contributed to the initiation and progression of atherosclerosis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cellules dendritiques
/
Facteur de transcription NF-kappa B
/
Système de signalisation des MAP kinases
/
Récepteurs éboueurs de classe E
/
Lipoprotéines LDL
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2021
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University/CN
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