Evaluation of Reverse Hybridization Diagnostic Strategy for Simultaneous Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae

ABSTRACT

We performed a non radioactive reverse hybridization assay for simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae ; two pathogens influencing humain immunodeficiency virus (HIV) sexual transmission ; using 200 endo-cervical samples from pregnant women (Institute Pasteur n Cote d'Ivoire ).Diagnosis DNA sequences were amplified by multiplex polymerase chain reaction (PCR)using biotinylated primers. Amplified products were hybridized to different specific probes fixed as a line on membrane strips and visualized using a straptavidine /alkaline phosphatase conjugated system. The results were compared to Elisa (Enzyme linked Immunosorben Assay ) techniques and culture .The overall prevalence was 5and 15for N. gonerrhoeae and C. tranchomatis respectively and specificity of the reserve hybridization assay was high(100) .Additionally; PCR and radioactive hybridization reported here showed an increase in the sensitivity for detection of trichomonas vaginalis over conventional methods .The multiplex assays were highly effective for simultaneous detection of C. tranchomatis and N.gonorrhoeae in endocervical swab samples from women with without clinical signs of genitourinary infection .This technique allowing simultaneous diagnosis of pathogens could be great importance for epidemiological studies and management in public health interventions: