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Cloning and Expression of NADPH-cytochrome P450 Reductase Gene in Chinese Mitten Crab, Eriocheir sinensis
Gao, Xianggang; Xia, Ying; Li, Yulong; He, Chongbo; Bao, Xiangbo; Liu, Weidong.
  • Gao, Xianggang; Key Lab of Marine Fishery Molecular Biology of Liaoning Province. Liaoning Ocean and Fisheries Science Research Institute. Dalian Liaoning. CN
  • Xia, Ying; Dalian Eco-Environmental Monitoring Center of Liaoning Province. Dalian Liaoning. CN
  • Li, Yulong; Key Lab of Marine Fishery Molecular Biology of Liaoning Province. Liaoning Ocean and Fisheries Science Research Institute. Dalian Liaoning. CN
  • He, Chongbo; Key Lab of Marine Fishery Molecular Biology of Liaoning Province. Liaoning Ocean and Fisheries Science Research Institute. Dalian Liaoning. CN
  • Bao, Xiangbo; Key Lab of Marine Fishery Molecular Biology of Liaoning Province. Liaoning Ocean and Fisheries Science Research Institute. Dalian Liaoning. CN
  • Liu, Weidong; Key Lab of Marine Fishery Molecular Biology of Liaoning Province. Liaoning Ocean and Fisheries Science Research Institute. Dalian Liaoning. CN
Braz. arch. biol. technol ; 64: e21210292, 2021. tab, graf
Article Dans Anglais | LILACS | ID: biblio-1278439
ABSTRACT
Abstract NADPH-cytochromeP450 reductase (CPR) is one of the most important components of the cytochrome P450 enzyme system. In this study, a gene encoding CPR (named EsCPR) was isolated from Eriocheir sinensis using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Analysis of the nucleotide sequence revealed a cDNA full-length of 3717 bp with an open reading frame of 2046 bp, a 5′-untranslated region of 42 bp, and a long 3′-untryganslated region of 1628bp, which encodes a protein of 681 amino acids with a predicted molecular weight of 30.7 kDa and an estimated pI of 4.82. The mature peptide shares amino acid of E. sinensis identity 82 % - 89 % to the CPR from Penaeus vannamei and Chionoecetes opilio. Tissues and developmental stage-dependent expression of EsCPR mRNA was investigated by real-time quantitative PCR. EsCPR mRNA was markedly expressed in the hepatopancreas and stomach. These results would provide valuable information for further study on the interactions between CPR and cytochrome P450 enzyme systems.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: NADPH-ferrihemoprotéine reductase / Clonage d'organisme / Brachyura langue: Anglais Texte intégral: Braz. arch. biol. technol Thème du journal: Biologie Année: 2021 Type: Article Pays d'affiliation: Chine Institution/Pays d'affiliation: Dalian Eco-Environmental Monitoring Center of Liaoning Province/CN / Key Lab of Marine Fishery Molecular Biology of Liaoning Province/CN

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: NADPH-ferrihemoprotéine reductase / Clonage d'organisme / Brachyura langue: Anglais Texte intégral: Braz. arch. biol. technol Thème du journal: Biologie Année: 2021 Type: Article Pays d'affiliation: Chine Institution/Pays d'affiliation: Dalian Eco-Environmental Monitoring Center of Liaoning Province/CN / Key Lab of Marine Fishery Molecular Biology of Liaoning Province/CN