Discovery of the ARP2 protein as a determining molecule in tumor cell death
Gac. méd. Méx
;
155(5): 504-510, Sep.-Oct. 2019. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1286551
ABSTRACT
Cancer is a multifactorial disease that constitutes a serious public health problem worldwide. Prostate cancer advanced stages are associated with the development of androgen-independent tumors and an apoptosis-resistant phenotype that progresses to metastasis. By studying androgen-independent lymphoid nodule carcinoma of the prostate (LNCaP) cells induced to apoptosis by serum elimination, we identified the activation of a non-selective cationic channel of 23pS conductance that promotes incoming Ca2+ currents, as well as apoptosis final stages. arp2cDNA was isolated and identified to be of the same cell type, and mRNA was expressed in Xenopus laevis oocytes, which was found to be associated with the activation of incoming Ca2+ currents and induction to apoptosis. cDNA, which encodes the ARP2 protein, was overexpressed in LNCaP cells and Chinese hamster ovary cells, which induced apoptosis. Our evidence suggests that protein ARP2 overexpression and transit to the cell membrane allows an increased Ca2+ incoming current that initiates the apoptosis process in epithelial-type cells whose phenotype shows resistance to programmed cell death.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tumeurs de la prostate
/
Calcium
/
Apoptose
/
Protéines régulatrices de l'apoptose
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Gac. méd. Méx
Thème du journal:
Médicament
Année:
2019
Type:
Article
Pays d'affiliation:
Mexique
Institution/Pays d'affiliation:
Universidad Nacional Autónoma de México/MX
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