Edaravone improves the post-traumatic brain injury dysfunction in learning and memory by modulating Nrf2/are signal pathway
Clinics
;
76: e3131, 2021. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1350610
ABSTRACT
OBJECTIVES:
To investigate the molecular mechanism of edaravone (EDA) in improving the post-traumatic brain injury (TBI) dysfunction in learning and memory.METHODS:
In vitro and in vivo TBI models were established using hydrogen peroxide (H2O2) treatment for hippocampal nerve stem cells (NSCs) and surgery for rats, followed by EDA treatment. WST 1 measurement, methylthiazol tetrazolium assay, and flow cytometry were performed to determine the activity, proliferation, and apoptosis of NSCs, and malondialdehyde (MDA), lactic dehydrogenase (LDH), and reactive oxygen species (ROS) detection kits were used to analyze the oxides in NSCs.RESULTS:
Following EDA pretreatment, NSCs presented with promising resistance to H2O2-induced oxidative stress, whereas NSCs manifested significant increases in activity and proliferation and a decrease in apoptosis. Meanwhile, for NSCs, EDA pretreatment reduced the levels of MDA, LDH, and ROS, with a significant upregulation of Nrf2/antioxidant response element (ARE) signaling pathway, whereas for EDA-treated TBI rats, a significant reduction was observed in the trauma area and injury to the hippocampus, with improvement in memory and learning performance and upregulation of Nrf2/ARE signaling pathway.CONCLUSIONS:
EDA, by regulating the activity of Nrf2/ARE signal pathway, can improve the TBI-induced injury to NSCs and learning and memory dysfunction in rats.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Éléments de réponse aux anti-oxydants
/
Lésions traumatiques de l'encéphale
/
Édaravone
/
Apprentissage
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Clinics
Thème du journal:
Médicament
Année:
2021
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Affiliated Hospital of Hebei University of Engineering/CN
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