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Low-dose paclitaxel modulates the cross talk between the JNK and Smad signaling in primary biliary fibroblasts
Lu, Jiamei; Yu, Liang; Shi, Jianhua.
Affiliation
  • Lu, Jiamei; Hospital of Xian Jiaotong University. Department of Nephrology. Xian. CN
  • Yu, Liang; Hospital of Xian Jiaotong University. Department of Hepatobiliary Surgery. Xian. CN
  • Shi, Jianhua; Hospital of Xian Jiaotong University. Department of Hepatobiliary Surgery. Xian. CN
Rev. Assoc. Med. Bras. (1992, Impr.) ; Rev. Assoc. Med. Bras. (1992, Impr.);68(2): 159-164, Feb. 2022. tab, graf
Article de En | LILACS | ID: biblio-1365364
Bibliothèque responsable: BR1.1
ABSTRACT
SUMMARY

OBJECTIVE:

The objective of this study was to explore the molecular mechanism underlying the occurrence of benign bile duct stricture and the target of low-dose paclitaxel in the prevention of benign bile duct stricture.

METHODS:

Under the stimulation of transforming growth factor beta 1, the expression of collagen type I and connective tissue growth factor were detected on isolated primary fibroblasts. The phosphorylation levels of JNK and Smad2L were detected using Western blot. The effect of low-dose paclitaxel on the transforming growth factor beta 1-induced inhibition of type I collagen and connective tissue growth factor expression and JNK and Smad2L phosphorylation was also observed.

RESULTS:

Transforming growth factor beta 1 induced the secretion of type I collagen and connective tissue growth factor as well as JNK phosphorylation in biliary fibroblasts. The JNK inhibitor or siRNA-Smad2 inhibited the transforming growth factor beta 1-induced secretion of type I collagen and connective tissue growth factor. Low-dose paclitaxel inhibited the expression of type I collagen induced by transforming growth factor beta 1 and may inhibit the secretion of collagen in biliary fibroblasts.

CONCLUSION:

The activation of JNK/Smad2L induced by transforming growth factor beta 1 is involved in the occurrence of benign bile duct stricture that is mediated by the overexpression of type I collagen and connective tissue growth factor, and low-dose paclitaxel may inhibit the phosphorylation of JNK/Smad2L.
Sujet(s)
Mots clés

Texte intégral: 1 Indice: LILACS Sujet Principal: Paclitaxel Limites du sujet: Humans langue: En Texte intégral: Rev. Assoc. Med. Bras. (1992, Impr.) Thème du journal: Educa‡Æo em Sa£de / GestÆo do Conhecimento para a Pesquisa em Sa£de / MEDICINA Année: 2022 Type: Article

Texte intégral: 1 Indice: LILACS Sujet Principal: Paclitaxel Limites du sujet: Humans langue: En Texte intégral: Rev. Assoc. Med. Bras. (1992, Impr.) Thème du journal: Educa‡Æo em Sa£de / GestÆo do Conhecimento para a Pesquisa em Sa£de / MEDICINA Année: 2022 Type: Article