A Selective Fluorescent Probe for the Determination and Imaging of Fluoride in Living Cells
Braz. J. Pharm. Sci. (Online)
;
58: e18674, 2022. graf
Article
Dans Anglais
| LILACS
| ID: biblio-1374556
ABSTRACT
Abstract Fluoride anions are indispensable trace elements required for sustaining life. To investigate the homeostasis and action of fluoride in the body, a new highly sensitive and selective fluorescence detection method was designed for fluoride in aqueous solutions. A fluorescent probe for fluoride (FP-F) was synthesized for imaging F- in living cells. The design strategy for the probe was based on the specific reaction between fluoride and silica to mediate deprotection of this probe to fluorescein. Upon treatment with F-, FP-F, a closed and weakly fluorescent lactone, was transformed into an open and strongly fluorescent product. Under the optimum conditions, the detection limit for fluoride was 0.526 nM. FP-F could detect micromolar changes in F- concentrations in living cells by confocal microscopy.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Fluorescéine
/
Fluorescence
/
Fluor
langue:
Anglais
Texte intégral:
Braz. J. Pharm. Sci. (Online)
Thème du journal:
Farmacologia
/
Teraputica
/
Toxicologia
Année:
2022
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Institute of Shandong Xinhua Pharmaceutical Co., Ltd/CN
/
Ludong University/CN
/
Shandong College Of Traditional Chinese Medicine/CN
/
Shandong College of Traditional Chinese Medicine/CN
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