Oocyte quality assessment in marine invertebrates: a novel approach by fluorescence spectroscopy
Biol. Res
;
55: 34-34, 2022. ilus, tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-1403572
ABSTRACT
BACKGROUND:
The assessment of oocyte quality is, nowadays, a major challenge in aquaculture, oocyte cryopreservation, and environmental science. Oocyte quality is a determining factor in fertilization and embryo development; however, there is still a lack of rapid and sensitive cellular markers for its assessment. Currently, its estimation is pre-dominantly based on morphological analysis, which is subjective and does not consistently reflect the developmental competence of the oocytes. Despite several recent studies investigating molecular markers related to oocyte quality, methods currently available for their determination pose various technical challenges and limitations. In this study, we developed a novel approach based on fluorescence spectroscopy to assess different intrinsic physiological parameters that can be employed to evaluate egg quality in marine invertebrates that are widely used as animal models such as sea urchins and mussels.RESULTS:
Different physiological parameters, such as viability, mitochondrial activity, intracellular ROS levels, plasma membrane lipid peroxidation, and intracellular pH, for egg quality evaluation have been successfully assessed in sea urchins and mussels by using specific fluorescent dyes and detecting the fluorescent signals in eggs through fluorescence spectroscopy.CONCLUSIONS:
Based on our findings, we propose these physiological markers as useful predictors of egg quality in marine invertebrates; they can be estimated rapidly, selectively, and sensitively by employing this novel approach, which, due to the speed of analysis, the low cost, and easy use can be considered a powerful analytical tool for the egg quality assessment.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Ovocytes
/
Développement embryonnaire
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2022
Type:
Article
Pays d'affiliation:
Italie
Institution/Pays d'affiliation:
Stazione Zoologica Anton Dohrn/IT
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