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Cytotoxicity Comparison of a Calcium Silicate-Based Resin Cement versus Conventional Self-Adhesive Resin Cement and a Resin-Modified Glass Ionomer: Cell Viability Analysis
Kashi, Faezeh; Abbasi, Mahdi; Ahmadi, Elham; Omrani, Ladan Ranjbar; Rafeiee, Niyousha; Kharazifard, Mohammad Javad.
  • Kashi, Faezeh; Guilan University of Medical Sciences. School of Dentistry. Department of Maxillofacial Radiology. Guilan. IR
  • Abbasi, Mahdi; Tehran University of Medical Sciences. School of Dentistry. Department of Operative Dentistry. Tehran. IR
  • Ahmadi, Elham; Tehran University of Medical Sciences. School of Dentistry. Department of Operative Dentistry. Tehran. IR
  • Omrani, Ladan Ranjbar; Tehran University of Medical Sciences. School of Dentistry. Department of Operative Dentistry. Tehran. IR
  • Rafeiee, Niyousha; Tehran University of Medical Sciences. School of Dentistry. Dentistry Research Institute. Tehran. IR
  • Kharazifard, Mohammad Javad; Tehran University of Medical Sciences. School of Dentistry. Dentistry Research Institute. Tehran. IR
Pesqui. bras. odontopediatria clín. integr ; 22: e210185, 2022. tab, graf
Article Dans Anglais | LILACS, BBO | ID: biblio-1422268
ABSTRACT
Abstract

Objective:

To compare the cytotoxicity level of a new calcium silicate-based resin cement (TheraCem) with two commonly used cements, including a conventional self-adhesive resin cement (Panavia SA) and a resinmodified glass ionomer cement (FujiCem2), on the human gingival fibroblast cells after 24 and 48 hours. Material and

Methods:

Twelve discs of each cement type were fabricated. The extract of cement disks was made by incubating them in the cell medium. Human gingival fibroblast cells were cultured and exposed to cement extracts for 24 h and 48 h. MTT assay was performed on extracts and optical density and cell viability rates were calculated by the spectrophotometer device at 570 nm. Data were analyzed using ANOVA and Tukey HSD tests.

Results:

The cell viability rates after 24 hours and 48 hours were as follows TheraCem 89.24% and 85.46%, Panavia SA 49.51% and 46.57% and FujiCem2 50.63% and 47.36%. TheraCem represented the highest cell viability rate. However, no significant difference was noted between Panavia SA and FujiCem2. Time had no significant effect on cell viability.

Conclusion:

TheraCem exhibited the best results among three tested cements and was considered non-toxic. Panavia SA and FujiCem2 were not significantly different regarding the cell viability rate. Time had no significant effect on the cytotoxicity level of cements (AU).
Sujets)


Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Calcarea Silicata / Céments résine / Fibroblastes / Ciment ionomère au verre langue: Anglais Texte intégral: Pesqui. bras. odontopediatria clín. integr Thème du journal: Dentisterie Année: 2022 Type: Article Pays d'affiliation: Iran Institution/Pays d'affiliation: Guilan University of Medical Sciences/IR / Tehran University of Medical Sciences/IR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Calcarea Silicata / Céments résine / Fibroblastes / Ciment ionomère au verre langue: Anglais Texte intégral: Pesqui. bras. odontopediatria clín. integr Thème du journal: Dentisterie Année: 2022 Type: Article Pays d'affiliation: Iran Institution/Pays d'affiliation: Guilan University of Medical Sciences/IR / Tehran University of Medical Sciences/IR