High microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristics
Braz. j. otorhinolaryngol. (Impr.)
;
89(2): 254-263, March-Apr. 2023. tab, graf
Article
Dans Anglais
|
LILACS-Express
| LILACS
| ID: biblio-1439716
ABSTRACT
Abstract Objectives:
To evaluate the bacterial microbiome found in tracheostomy cannulas of a group of children diagnosed with glossoptosis secondary to Robin Sequence (RS), and its clinical implications.Methods:
Pediatric patients were enrolled in the study at the time of the cannula change in the hospital. During this procedure, the removed cannula was collected and stored for amplicon sequencing of 16s rRNA. DNA extraction was performed using DNeasy PowerBiofilm Kit (QIAGEN® - Cat nº 24000-50) while sequencing was performed with the S5 (Ion S5™ System, Thermo Fisher Scientific), following Brazilian Microbiome Project (BMP) protocol.Results:
All 12 patients included in the study were using tracheostomy uncuffed cannulas of the same brand, had tracheostomy performed for over 1-year and had used the removed cannula for approximately 3-months. Most abundant genera found were Aggregatibacter, Pseudomonas, Haemophilus, Neisseria, Staphylococcus, Fusobacterium, Moraxella, Streptococcus, Alloiococcus, and Capnocytophaga. Individual microbiome of each individual was highly variable, not correlating to any particular clinical characteristic.Conclusion:
The microbiome of tracheostomy cannulas is highly variable, even among patients with similar clinical characteristics, making it challenging to determine a standard for normality. © 2022 Associa¸c˜ao Brasileira de Otorrinolaringologia e Cirurgia C´ervico-Facial. Published by Elsevier Editora Ltda. This is an open access article under the CC BY license (https//creativecommons.org/licenses/by/4.0/).
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Type d'étude:
Guide de pratique
langue:
Anglais
Texte intégral:
Braz. j. otorhinolaryngol. (Impr.)
Thème du journal:
Otolaryngologie
Année:
2023
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Hospital de Clínicas de Porto Alegre (HCPA)/BR
/
Universidade Federal do Rio Grande do Sul (UFRGS)/BR
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