In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations
Acta sci., Biol. sci
;
43: e52866, 2021. tab
Article
Dans Anglais
| LILACS, VETINDEX
| ID: biblio-1460986
ABSTRACT
In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
Manihot
/
Acides naphtalèneacétiques
Type d'étude:
Guide de pratique
langue:
Anglais
Texte intégral:
Acta sci., Biol. sci
Année:
2021
Type:
Article
Institution/Pays d'affiliation:
Empresa Brasileira de Pesquisa Agropecuária/BR
/
Faculdade Maria Milza/BR
/
Universidade Federal do Recôncavo da Bahia/BR
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