WD repeat domain 5 promotes the development of late-onset preeclampsia by activating nuclear factor kappa B
Acta cir. bras
;
38: e386223, 2023. tab, graf, ilus
Article
Dans Anglais
| LILACS, VETINDEX
| ID: biblio-1527603
ABSTRACT
Purpose:
Over-activation of nuclear factor kappa B (NF-κB) was proven to be involved in the pathogenesis of preeclampsia. However, its regulation mechanism is not clear yet. This paper explored the role of WD repeat domain 5 (WDR5) in the development of late-onset preeclampsia and its relationship with NF-κB.Methods:
WDR5 expression was detected in normal placentas and placentas from late-onset preeclampsia patients. CCK-8 and colony formation assays were conducted to appraise the proliferative ability of trophoblast. Migration and invasion were observed by wound healing and transwell assays. The interaction between WDR5 and NF-κB inhibitor I-kappa-B-alpha (IkBa) was verified by Co-immunoprecipitation analysis. Immunofluorescence was used to analyze the activation of NF-κB. Finally, we tested the role of WDR5 using the mice late-onset preeclampsia model.Results:
WDR5 was highly expressed in the placentas of late-onset preeclampsia patients. WDR5 overexpression suppressed cell proliferation, migration, and invasion in trophoblast. WDR5 could interact with IkBa to activate NF-κB. Knockdown of NF-κB counteracted the anti-proliferative and anti-metastatic effects of WDR5 overexpression in trophoblast. In-vivo studies suggested that targeting WDR5 combated late-onset preeclampsia development.Conclusions:
Our finding provides new insights into the role of WDR5 in late-onset preeclampsia development.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Pré-éclampsie
/
Trophoblastes
/
Facteur de transcription NF-kappa B
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2023
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Liaocheng Peoples Hospital/CN
/
Shandong Provincial Hospital Affiliated to Shandong First Medical University/CN
/
The Affiliated Taian City Central Hospital of Qingdao University/CN
/
The Second Affiliated Hospital of Shandong First Medical University/CN
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