Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis
Rev. Inst. Med. Trop. São Paulo (Online)
;
66: e22, 2024. tab, graf
Article
Dans Anglais
|
LILACS-Express
| LILACS
| ID: biblio-1559120
ABSTRACT
ABSTRACT Noroviruses are highly infectious, genetically diverse viruses. Global outbreaks occur frequently, making molecular surveillance important for infection monitoring. This cross-sectional descriptive study aimed to monitor cases of norovirus gastroenteritis in the Brazilian Amazon. Fecal samples were tested by immunoenzymatic assay, RT-PCR and genetic sequencing for the ORF1/ORF2 and protease regions. Bayesian inference with a molecular clock was employed to construct the phylogeny. The norovirus prevalence was 25.8%, with a higher positivity rate among children aged 0-24 months. Genogroup GII accounted for 98.1% of the sequenced samples, while GI accounted for 1.9% of them. The GII.P16/GII.4 genotype was the most prevalent, with an evolution rate of 2.87x10−3 and TMRCA estimated in 2012. This study demonstrates that norovirus is a primary causative agent of gastroenteritis and provides data on viral genetic diversity that may facilitate infection surveillance and vaccine development.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Rev. Inst. Med. Trop. São Paulo (Online)
Thème du journal:
Medicina Tropical
Année:
2024
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Instituto Evandro Chagas/BR
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