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Inhibition of HMG-CoA reductase activity and cholesterol permeation through Caco-2 cells by caffeoylquinic acids from Vernonia condensata leaves
Arantes, Ana A.; Falé, Pedro L.; Costa, Larissa C.B.; Pacheco, Rita; Ascensão, Lia; Serralheiro, Maria Luísa.
  • Arantes, Ana A.; Universidade de Lisboa. Faculdade de Ciências. Centro de Química e Bioquímica. Lisboa. PT
  • Falé, Pedro L.; Universidade de Lisboa. Faculdade de Ciências. Centro de Química e Bioquímica. Lisboa. PT
  • Costa, Larissa C.B.; Universidade de Lisboa. Faculdade de Ciências. Centro de Química e Bioquímica. Lisboa. PT
  • Pacheco, Rita; Universidade de Lisboa. Faculdade de Ciências. Centro de Química e Bioquímica. Lisboa. PT
  • Ascensão, Lia; Universidade de Lisboa. Faculdade de Ciências. Centro de Química e Bioquímica. Lisboa. PT
  • Serralheiro, Maria Luísa; Universidade de Lisboa. Faculdade de Ciências. Centro de Química e Bioquímica. Lisboa. PT
Rev. bras. farmacogn ; 26(6): 738-743, Nov.-Dec. 2016. tab, graf
Article Dans Anglais | LILACS | ID: biblio-829908
ABSTRACT
ABSTRACT The aim of this study was to provide scientific knowledge to support the use of Vernonia condensata Baker, Asteraceae, beverages for their alleged hypocholesterolemic properties by testing their action as HMG-CoA reductase inhibitors and their capacity to lower dietary cholesterol permeation. Chlorogenic acid, and other caffeoylquinic acids derivatives were identified as the main components of these beverages by LC–MS/MS. No changes in the composition were notice after the in vitro gastrointestinal digestion and no toxicity against Caco-2 and HepG2 cell lines was detected. Cholesterol permeation through Caco-2 monolayers was reduced in 37% in the presence of these herbal teas, and the caffeoylquinic acids permeated the monolayers in 30–40% of their initial amount in 6 h. HMG-CoA reductase activity was reduced with these beverages, showing an IC50 of 217 µg ml−1. It was concluded that caffeoylquinic acids, the major components, justified 98% of the enzyme inhibition measured.


Texte intégral: Disponible Indice: LILAS (Amériques) langue: Anglais Texte intégral: Rev. bras. farmacogn Thème du journal: Pharmacie Année: 2016 Type: Article Pays d'affiliation: Portugal Institution/Pays d'affiliation: Universidade de Lisboa/PT

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Texte intégral: Disponible Indice: LILAS (Amériques) langue: Anglais Texte intégral: Rev. bras. farmacogn Thème du journal: Pharmacie Année: 2016 Type: Article Pays d'affiliation: Portugal Institution/Pays d'affiliation: Universidade de Lisboa/PT